EGFR pTyr1173 Mouse Monoclonal Antibody [Clone ID: 9H2]

Specifications

Product Data
Clone Name	9H2
Applications	IF
Recommend Dilution	Immunocytochemistry: Use at 1-10 μg/ml. For ELISA, Immunoprecipitation and Immunoblotting, use Purified antibody (Cat.-No. AM00042PU-N) or Biotinylated antibody (Cat.-No. AM00042BT-N).
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH. Epitope: Phospho-Tyr1197 N A E pY L R V
Specificity	This antibody specifically interacts with the 1197 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR. Antibody AM00042FC-N does not crossreact with the highly homologous pTyr1248 of acticated erbB2.
Formulation	PBS containing 0.09% Sodium Azide/PEG and Sucrose. Label: FITC State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	FITC
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.