EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]

Specifications

Product Data
Clone Name	20E12
Applications	WB
Recommend Dilution	Western blot (0.5 µg/ml for HRPO/ECL detection; recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin, extracellular domain
Specificity	This antibody specifically recognizes the extracellular domain of EGF receptor.
Formulation	PBS, 0.09 % Na-azide, PEG and Sucrose State: Purified State: Lyophilized Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent ultrafiltration and size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze in liquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGF Receptor (EGFR) and erbB2, erbB3, and ErbB4 are members of subclass I of receptor tyrosine kinases. EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight: 180 kDA. Positive control included: Cell lysate from untreated A431 cells.
Reference Data

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