EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]

Specifications

Product Data
Clone Name	13G8
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA: 0.05 μg/ml. Western Blot: 1 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunoprecipitation: 1-10 µg per 106 pervanadate-treated A431 cells. Immunocytochemistry: 1-10 μg/ml. Luminex.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH. Epitope: C-terminus (aa 1165-1186), independent of phosphorylation status.
Specificity	This antibody specifically recognizes the C-terminus of EGF receptor (aa 1165-1186). Recognition is independent of the phosphorylation status at tyrosine 1173.
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose Label: Biotin State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	180 kDa
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGF Receptor (EGFR) and erbB2, erbB3, and ErbB4 are members of subclass I of receptor tyrosine kinases. EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Included Positive Control: Cell lysate from untreated HepG2 cells Protocol: Positive Control: Cell lysate from untreated HepG2 cells. Formulation: Lyophilized Cell lysate from serum starved HepG2 cells Reconstitution: Reconstitute by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control is recommended for immunoblot applications. 20μl of Positive control cell lysate correspond to Ca. 80.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended fro applications with native proteins such as Immunoprecipitation. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch.
Reference Data

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