Her2 (ERBB2) (1240-1260) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19D2]

CAT#: AM00052BT-N

Her2 (ERBB2) (1240-1260) (incl. pos. control) mouse monoclonal antibody, clone 19D2, Biotin

Conjugation: Unconjugated Biotin



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CNY 6,798.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 19D2
Applications WB
Recommend Dilution Immunoblotting: 1 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for more details).
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Peptide conjugated to hemocyanin
Epitope: aa 1240-1260
Specificity This antibody specifically recognizes EerbB2 at 185 kDa.
Formulation PBS, 0.09% Sodium Azide/PEG and Sucrose.
Label: Biotin
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation Biotin
Storage Condition Aliquote and freeze in liquid nitrogen
Antibody can be stored frozen at -80°C up to 1 year.
Thaw aliquots at 37°C.
Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name erb-b2 receptor tyrosine kinase 2
Background ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.
Synonyms HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
Note Mol. weight: 185 kDa.

Protocol: Positive Control Provided.
Cell lysate from untreated SKOV-3


Description: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)

Format: Lyophilized cell lysate from serum starved SKOV-3 cells.

Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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