ErbB 3 (ERBB3) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11A4]

Specifications

Product Data
Clone Name	11A4
Applications	WB
Recommend Dilution	Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated SW620 cells (See Protocols).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide hemocyanin conjugated derived from C-terminus of erB3/HEr3
Specificity	This antibody specifically recognizes the C-terminus of erbB3 at 185 kDa.
Formulation	1ml PBS State: Purified State: Lyophilized purified IgG fraction Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml H2O (15 min, RT)
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	185 kDa
Gene Name	erb-b2 receptor tyrosine kinase 3
Database Link	Entrez Gene 2065 Human UniProt ID P21860
Background	ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsic kinase activity due to an amino acid substitution within the ATP binding site. Upon heterodimerization with the other erbB family members, erbB3 becomes phosphorylated at multiple tyrosine residues that represent docking sites for downstream signaling proteins, e.g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.
Synonyms	ERBB-3, c-erbB-3, HER-3
Reference Data

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