ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C5]

Specifications

Product Data
Clone Name	6C5
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Positive Control: Cell lysate from untreated T47D cells (please see "Protocols" for details).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin. Epitope: Amino acids 1230-1250
Specificity	This antibody specifically recognizes erbB4 at 180 kDa.
Formulation	1 ml PBS containing 0.09% Sodium Azide as preservative, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	erb-b2 receptor tyrosine kinase 4
Database Link	Entrez Gene 2066 Human UniProt ID Q15303
Background	erbB4/Her4 is a member of the receptor tyrosine kinase subfamily that includes EGFR, erbB2/Her2, and erbB3/Her3. erbB4 is a receptor for Neuregulin 1 (NRG1) and regulates cell proliferation and differentiation.
Synonyms	p180erbB4, c-ErbB-4, HER-4
Note	Protocol: Positive Control: Cell lysate from T47D untreated Lot No. 101101 Format: Lyophilized cell lysate from Serum starved T47D cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing
Reference Data

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