FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]

Specifications

Product Data
Clone Name	6B9
Applications	ELISA, WB
Recommend Dilution	ELISA: 0.1 µg/ml. Immunoblotting: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated T47D cells (See Protocol below).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.
Specificity	This antibody specifically recognizes fer at 90 kDa.
Formulation	1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT)
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	FER tyrosine kinase
Database Link	Entrez Gene 2241 Human UniProt ID P16591
Background	Fer is a non-receptor tyrosine kinase that is widely expressed in different tissues and cell lines. Fer is a homologue of the FES tyrosine kinase that is primarily expressed in cells of hematopoietic origin.
Synonyms	c-FER, p94-FER, Tyrosine kinase 3
Note	Protocol: Positive Control: Cell lysate from untreated T47D cells. Format: Lyophilized cell lysate from serum starved T47D cells. Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data

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