c Fos (FOS) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8B5]

Specifications

Product Data
Clone Name	8B5
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 0.1 µg/ml (protein ELISA). Immunohistochemistry on Frozen Sections. Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated HepG2 cells (See 'Protocols').
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.
Specificity	This antibody specifically interacts with the N-terminus of c-Fos. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.
Formulation	2x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	Fos proto-oncogene, AP-1 transcription factor subunit
Database Link	Entrez Gene 2353 Human UniProt ID P01100
Background	The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.
Synonyms	FOS, G0S7
Note	Protocol: Positive Control: Cell lysate from untreated HepG2 cells. Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data

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