IGF1 Receptor (IGF1R) pTyr1316, (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2B9]

Specifications

Product Data
Clone Name	2B9
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control Included: Cell lysate form Insulin-treated HEK-293 cells. (Please see also  "Protocols"). ELISA: 0.1 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH.
Specificity	This antibody specifically recognizes the IGF1 receptor phosphorylated at tyrosine 1316.
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	insulin like growth factor 1 receptor
Database Link	Entrez Gene 3480 Human UniProt ID P08069
Background	The IGF1 receptor (IGF1R) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The IGF1 receptor is activated upon binding of the peptide hormones IGF1 and IGF2, leading to autophosphorylation of tyrosine residues 1131, 1135, and 1136 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 950 and 1316 regulate the assembly of signal tranduction complexes.
Synonyms	IGF-I receptor, IGF1 Receptor
Note	Protocol: Positive Control Cell Lysate, Insulin-treated Formulation: Lyophilized cell lysate from HEK-293 cells. Serum starved cells were treated for 15 min with insulin. Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. (Aliquote and store frozen. Avoid repeated freeze/thaw cycles). Application: The positive cell lysate is recommended for immunoblot applications. 20 µl of positive cell lysate correspond to ca. 20.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of target proteins. Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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