JNK2 (MAPK9) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]

Specifications

Product Data
Clone Name	12C5
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 g/ml. Immunohistochemistry. Included Positive Control: Cell lysate from untreated A431 cells (See Protocol).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH. Epitope: N-terminus
Specificity	This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.
Formulation	1 ml PBS containing 0.09% Sosium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent thiophilic adsorbtion and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	54 kDa
Gene Name	mitogen-activated protein kinase 9
Database Link	Entrez Gene 5601 Human UniProt ID P45984
Background	Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1.
Synonyms	JNK-55, PRKM9, MAP kinase 9
Note	Protocol: Positive Control Cell Lysate (A431 Untreated) Formulation: Lyophilized cell lysate from serum starved A431 Cells. Reconstitution: Restore by Addition of 200 µl Water. After complete solubilization Add 200 µl 2x SDS-PAGE Sample Buffer, mix and incubate at 90°C for 5 mn. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. Application: The Positive Control is recommended for Immunoblot (20 µl of Positive Control Cell lysate corresponds to ~ 20.000 Cells). Use µl /lane (mini gel) for HRPO/ECL detection of the target proteins. Please Note: The lyophilized cell lysates contain SDS and are not recomended for the applications with native proteins such as Immunoprecipitation.
Reference Data

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