ERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12D4]

Specifications

Product Data
Clone Name	12D4
Applications	IF
Recommend Dilution	Immunocytochemistry: 1 - 10 µg/ml.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH. Epitope: ...pT-E-pY...
Specificity	This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). T he antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose Label: FITC State: Liquid purified IgG
Concentration	lot specific
Purification	Subsequent thiophilic adsorption and size exclusion chromatography
Conjugation	FITC
Storage Condition	Aliquote and freeze in liquid nitrogen. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase 3
Database Link	Entrez Gene 5595 Human UniProt ID P27361
Background	Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
Synonyms	MAP kinase 3, MAPK 3, ERK-1, ERT2, p44-MAPK, p44-ERK1, PRKM3
Reference Data

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