ERK2 (MAPK1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6G11]
CAT#: AM00085BT-N
ERK2 (MAPK1) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 6G11, Biotin
Conjugation: Unconjugated
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CNY 5,973.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | 6G11 |
Applications | ELISA, IP, WB |
Recommend Dilution | ELISA: Use at 0.05 µg/ml. Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: 1-10 µg/ml per 10e6 pervanadate-treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details). |
Reactivity | Canine, Human, Mouse, Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Peptide conjugated to KLH Epitope: C-terminus |
Specificity | This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1). |
Formulation | PBS with 0.09% Sodium Azide/PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Subsequent Thiophilic Adsorption and Size Exclusion Chromatography. |
Conjugation | Biotin |
Storage Condition | Aliquote and freeze in liquid nitrogen Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
Gene Name | mitogen-activated protein kinase 1 |
Database Link | |
Background | Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y. |
Synonyms | Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1 |
Note | Mol. weight: 42 kDa Protocol: Positive Control Provided. Cell lysate from untreated HepG2 Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human) Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. |
Reference Data | |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway |
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