malE Mouse Monoclonal Antibody [Clone ID: 6E4]

Specifications

Product Data
Clone Name	6E4
Applications	ELISA, WB
Recommend Dilution	Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Escherichia coli
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified MBP (Maltose Binding Protein)
Specificity	This antibody can be used for the detection of MBP fusion proteins.
Formulation	1ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT)
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid Nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Predicted Protein Size	43 kDa
Database Link	UniProt ID P0AEX9
Background	Maltose Binding Protein (MBP), the E. coli malE gene product, is widely used as fusion partner for the expression of recombinant proteins.
Synonyms	MMBP, Maltodextrin-binding protein, malE-Tag
Reference Data

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