MEK2 (MAP2K2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8E8]

Specifications

Product Data
Clone Name	8E8
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 µg/ml (protein ELISA).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH
Specificity	This antibody specifically recognizes the N-terminus of MEK2 at 45 kDa.
Formulation	1 ml PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT)
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase kinase 2
Database Link	Entrez Gene 5605 Human UniProt ID P36507
Background	MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
Synonyms	MAP kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MKK2, MAP kinase kinase 2
Reference Data

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