PIN1 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8C10]

Specifications

Product Data
Clone Name	8C10
Applications	ELISA, WB
Recommend Dilution	ELISA: 0.1 µg/ml (protein ELISA).  Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.  Included Positive Control: Cell lysate from untreated HepG2 cells.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human PIN1
Specificity	This antibody specifically recognizes Human PIN1 in cell extracts at 16 kDa.
Formulation	1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Database Link	Entrez Gene 5300 Human UniProt ID Q13526
Background	PIN1 is a peptidyl-prolyl-cis-trans-isomerase (PPIase) that specifically interacts with serine phosphate-proline or threonine phosphate-proline motifs. Upon binding, PIN1 isomerizes the peptide bond form cis to trans. Known substrates of PIN1 are serveral mitotic phosphoproteins (e.g. cdc25) as well as phosphorylated p53, phosphorylated β-catenin and phosphorylated tau protein. It is assumed that the isomerization of phosphoproteins regulates their biological function.
Synonyms	PIN-1
Reference Data

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