AKT1 pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11E6]

Specifications

Product Data
Clone Name	11E6
Applications	ELISA, WB
Recommend Dilution	ELISA: Use at 0.05 μg/ml. Immunoblotting (Western): 0.5 µg/ml for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Positive Control: Cell lysate from pervanadate treated HepG2 cells.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH. Epitope: phosphoserine 473 P Q F pS Y S A
Specificity	Clone 11E6 specifically recognizes PKB phosphorylated at serine 473.
Formulation	1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze / thaw cycles.
Gene Name	AKT serine/threonine kinase 1
Database Link	Entrez Gene 207 Human UniProt ID P31749
Background	The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. Activated PKB is phosphorylated at serine 473 (mTOR/Rictor or DNA-PK) and threonine 308 (PDK1).
Synonyms	Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT
Note	A Positive Control cell lysate is provided (please see "protocols" for details). Protocol: Positive Control: Cell lysate from HepG2 pervanadate treated Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. with pervanadate Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing
Reference Data

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