Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2C8]

Specifications

Product Data
Clone Name	2C8
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1 - 10 µg per 106 pervanadate-treated A431 cells.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH
Specificity	Mab PTYR-2C8 recognizes a broad range of tyrosine--phosphorylated proteins in crude cell extracts and may therefore be particularly well-suited for the detection/screening of tyrosine phosphorylated proteins.
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified Ig
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Background	Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Note	Includes positive control (see protocols). Protocol: Phosphotyrosine MW standard Size: 20 Blots Formulation: Phosphotyrosine modified standard proteins lyophilized from PBS / 0.1 % SDS / PEG / Sucrose / Malachitgreen. The following standard proteins were modified with phosphotyrosine: galactosidase (116 kD), phosphorylase A (98 kD), BSA (67 kD), ovalbumin (46 kD), carbonic anhydrase (32 kD), and soybean trypsin inhibitor (24 kD). Storage: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The phosphptyrosine molecular weight marker is recommended for immunoblot applications. Use 20 µl of the phosphotyrosine molecular weight marker per lane (mini gel). The individual proteins of the marker are recognized by the following commercially available clones: 2C8, 1F9, 3B12, 9F1, 9H8, 16F4.
Reference Data

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