Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E9]

Specifications

Product Data
Clone Name	1E9
Applications	WB
Recommend Dilution	Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.
Specificity	This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
Formulation	1 ml PBS containing 0.09% Sodium Azide as preservative, PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Gene Name	phospholipid scramblase 1
Database Link	Entrez Gene 5359 Human UniProt ID O15162
Background	Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.
Synonyms	Phospholipid scramblase 1, PL scramblase 1, MmTRA1b
Note	Protocol: Positive Control Cell Lysate HepG2 untreated Size Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency

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