SHC (SHC1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24E4]

Specifications

Product Data
Clone Name	24E4
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated Neuro 2A cells (See Protocol below).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.  Epitope: N-terminus.
Specificity	This antibody specifically recognizes the N- terminus of Shc/p66.
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose, 50% Glycerol State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	66 kDa
Gene Name	SHC adaptor protein 1
Database Link	Entrez Gene 6464 Human UniProt ID P29353
Background	Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.
Synonyms	SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA
Note	Protocol: Included Positive Control: Cell lysate from untreated Neuro 2A cells. Formulation: Lyophilized cell lysate from serum starved Neuro 2A cells. Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 54 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation! Storage: Aliqot and store at -20°C. Avoid repeated freezing and thawing.
Reference Data

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