VASP pSer157 Mouse Monoclonal Antibody [Clone ID: 5C6]

CAT#: AM00155PU-N

VASP pSer157 mouse monoclonal antibody, clone 5C6, Purified

Conjugation: Unconjugated Biotin FITC



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CNY 6,578.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

Product images

Specifications

Product Data
Clone Name 5C6
Applications ELISA, FC, IF, IP, WB
Recommend Dilution Western blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended Blocking Buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.05 µg/ml.
Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells.
Immunocytochemistry: 1-10 µg/ml (Monoclonal 5C6 antibody may tolerate 0.5% formaldehyde fixation).
Flow Cytometry.
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Epitope: Phosphoserine 157 RRVpSNAG
Specificity AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA).
The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
Formulation PBS with 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction from serum-free cell culture supernatant
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Gene Name vasodilator-stimulated phosphoprotein
Background VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines; paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.
Molecular Weight: 46/50 kDa
Synonyms Vasodilator-stimulated phosphoprotein
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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