VASP pSer157 Mouse Monoclonal Antibody [Clone ID: 5C6]

Specifications

Product Data
Clone Name	5C6
Applications	ELISA, FC, IF, IP, WB
Recommend Dilution	Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended Blocking Buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells. Immunocytochemistry: 1-10 µg/ml (Monoclonal 5C6 antibody may tolerate 0.5% formaldehyde fixation). Flow Cytometry.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH Epitope: Phosphoserine 157 RRVpSNAG
Specificity	AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
Formulation	PBS with 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction from serum-free cell culture supernatant
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	vasodilator-stimulated phosphoprotein
Database Link	Entrez Gene 7408 Human UniProt ID P50552
Background	VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines; paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Molecular Weight: 46/50 kDa
Synonyms	Vasodilator-stimulated phosphoprotein
Reference Data

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