VASP pSer239 Mouse Monoclonal Antibody [Clone ID: 16C2]

Specifications

Product Data
Clone Name	16C2
Applications	ELISA, FC, IF, IP, WB
Recommend Dilution	ELISA (0.05 µg/ml). Immunoblotting (0.5 µg/ml for HRPO/ECL detection). Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Flow Cytometry. Immunoprecipitation (1-10 µg per 10e6 pervanadate-treated A431 cells). Immunocytochemistry (1-10 µg/ml; may tolerate 0.5% formaldehyde fixation).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH Epitope: Phosphoserine 239 RKVpSKQE
Specificity	AM00156PU-N recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, mab AM00156PU-N (clone 16C2) is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.
Formulation	2x PBS containing 0.09% Sodium Azide, PEG and Sucrose. State: Purified State: Lyophilized purified Ig fraction from serum-free cell culture supernatant
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquot and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months. Avoid repeated freeze / thaw cycles!
Gene Name	vasodilator-stimulated phosphoprotein
Database Link	Entrez Gene 7408 Human UniProt ID P50552
Background	VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines; paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.
Synonyms	Vasodilator-stimulated phosphoprotein
Note	Molecular Weight: 46/50 kDa
Reference Data

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