Bovine Corona Virus E2 protein Mouse Monoclonal Antibody [Clone ID: 5A4]

CAT#: AM00683PU-N

Bovine Corona Virus E2 protein mouse monoclonal antibody, clone 5A4, Purified



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CNY 8,175.00


货期*
5周

规格
    • 1 mg

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Specifications

Product Data
Clone Name 5A4
Applications ELISA
Recommend Dilution ELISA.
HIT (hemagglutination inhibition titer).
Reactivity Bovine Coronavirus
Host Mouse
Clonality Monoclonal
Immunogen Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Specificity Recognizes bovine corona virus surface antigen (peplomer).
Formulation PBS, pH 7.4 containing 0.09% Sodium azide
State: Purified
State: Liquid purified IgG
Concentration lot specific
Purification >95% pure (SDS-PAGE). Protein G chromatography
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Background

Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.

S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Synonyms Spike glycoprotein, S glycoprotein, Peplomer protein, E2 protein
Note Centrifuge before opening to ensure complete recovery of vial contents.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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