Tfrc Rat Monoclonal Antibody [Clone ID: R17217.1.4]

CAT#: AM00702PU-S

Tfrc rat monoclonal antibody, clone R17217.1.4, Purified

Conjugation: Unconjugated FITC PE



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CNY 6,083.00


货期*
5周

规格
    • 200 ug

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Specifications

Product Data
Clone Name R17217.1.4
Applications FC
Recommend Dilution Flow Cytometry.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Specificity Anti-mouse CD71 monoclonal antibody reacts with the transferrin receptor (CD71). The expression of CD71 is up-regulated after B cell or T cell activation.
Formulation PBS containing 0.09% sodium azide (NaN3) as preservative
State: Purified
State: Liquid purified Ig fraction
Concentration lot specific
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name transferrin receptor
Synonyms TfR1, p90, Transferrin receptor protein 1
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A.For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add ~1.0 μg of this antibody.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 μl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1/500 dilution.
9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C in media B.
11. Resuspend the cell pellet in 50 μl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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