DHLAG (CD74) Mouse Monoclonal Antibody [Clone ID: Bu45]

Specifications

Product Data
Clone Name	Bu45
Applications	FC
Recommend Dilution	Flow Cytometry: 1/5; 10µl of the suggested working dilution to label 1x10e6 cells in 100µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	B lymphoblastoid cell line HFB1.
Specificity	This antibody recognises CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognises 33, 35 and 41 kD isoforms of CD74. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus. *Membrane permeabilisation may enhance CD74 staining on monocytes but is not required when staining lymphocytes.
Formulation	Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	CD74 molecule
Database Link	Entrez Gene 972 Human UniProt ID P04233
Background	The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes.
Synonyms	p33, DHLAG
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.