Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: Ki-67]

Specifications

Product Data
Clone Name	Ki-67
Applications	FC, IF, WB
Recommend Dilution	Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.<br> Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.
Reactivity	Bovine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Nuclei of the Hodgkin lymphoma cell line L428
Specificity	This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	marker of proliferation Ki-67
Database Link	Entrez Gene 4288 Human UniProt ID P46013
Background	Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Synonyms	Ki67 antigen, MKI67, Proliferation marker
Reference Data

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