Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: 2F5]

Specifications

Product Data
Clone Name	2F5
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product does not require pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections. Recommended Positive Control: Human spleen and lymph node.
Reactivity	Bovine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Superoxide dismutase enzyme prepared from bovine erythrocytes. Cells from immunised mice were fused with cells of the mouse Sp-2/0-Ag14 myeloma cell line.
Specificity	This antibody recognizes Copper/Zinc Superoxide Dismutase (SOD). It does not cross react with immunoglobulins but does react weakly with Mn SOD.
Formulation	PBS containing 0.09% Sodium Azide as preservative and 0.1% BSA as stabilizer State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	superoxide dismutase 1, soluble
Database Link	Entrez Gene 6647 Human UniProt ID P00441
Background	Superoxide dismutases are ubiquitous metalloproteins that destroy oxygen-mediated free radicals, that are normally produced within the cells, which are toxic to biological systems. There are three forms of superoxide dismutase, including the Fe, mitochondrial (Mn) and the copper/zinc binding (Cu/Zn) form. The Cu/Zn form of SOD is utilised by most eukaryotic organisms. SOD Cu/Zn prevents oxygen-mediated free radical damage by catalysing the dismutation of the toxic superoxide radical to molecular oxygen and hydrogen peroxide. Superoxide dismutatses catalyzes the conversion of single electron reduced species of molecular oxygen to hydrogen peroxide and oxygen. Cu, Zn superoxide dismutase (SOD1) is widely distributed and comprises 90% of total SOD. Mutations in SOD1 cause a form of familial ALS. Oxidative modifications and aggregation of SOD1 has been associated with Alzheimer’s and Parkinson's disease.
Synonyms	SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1
Reference Data

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