EMR3 (ADGRE3) Hamster Monoclonal Antibody [Clone ID: 3D7]

Specifications

Product Data
Clone Name	3D7
Applications	FC
Recommend Dilution	Flow cytometry ( Neat - 1/10): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivity	Human
Host	Hamster
Clonality	Monoclonal
Immunogen	ARHO-EMR3-CD97 (EGF1) transfectants. Spleen cells from immunised Armenian hamster were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognises epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), which is a 56kD member of the EGF-7 transmembrane (TM7) family of adhesion receptors.
Formulation	PBS, pH 7.4, containing 0.09% Sodium Azide Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer I
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	adhesion G protein-coupled receptor E3
Database Link	Entrez Gene 84658 Human UniProt ID Q9BY15
Background	EMR3 is expressed at the cell surface as a heterodimer. The molecule is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. Studies suggest that the EMR3 molecule is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells. The exact functions of EMR3 and its ligands have not yet been determined.
Reference Data

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