Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: 10-02A]

Specifications

Product Data
Clone Name	10-02A
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA: This antibody binds both C3c and C3 and so is not suitable for all ELISA applications. Western Blot. Flow Cytometry. Immunohistochemistry on frozen sections. Recommended Positive Control: Kidney from post streptococal glomerulonephritis patients.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Native human C3 from normal serum, highly purified by chromatography.
Specificity	This antibody recognises 135kDa complement component 3c (C3c), present in blood serum.
Formulation	Borate buffered saline pH 8.4 containing 0.02% Sodium Azide State: Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 3
Database Link	Entrez Gene 718 Human UniProt ID P01024
Background	C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha chain fragment 1 + alpha chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
Synonyms	CPAMD1, Complement component 3
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

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