Leptin (LEP) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human Leptin when used with Biotin conjugated anti-Human Leptin antibody (cat. PP1041B) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) recombinant Human Leptin
Specificity	This antibody detects Leptin.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody is stable at room temperature for one month or at -20°C for longer. Following reconstitution it is stable at 2-8°C for six weeks. Avoid repeated freezing and thawing.
Gene Name	leptin
Database Link	Entrez Gene 3952 Human UniProt ID P41159
Background	Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.
Synonyms	LEP, OB, OBS, Obesity factor, Obese protein
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.