TGF beta 1 (TGFB1) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGFB1 when used in conjunction with compatible secondary reagents.  Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a Dot Blot, a 1/500 dilution of this antibody exhibited complete cross reactivity with Human TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000 dilution. Immunohistochemistry on Paraffin Sections: 5.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an Alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (> 98%) Insect cells derived recombinant Human TGFB1 (insect derived)
Specificity	This antibody detects Human TGF-β1. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction from Tissue Culture Supernatant
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	transforming growth factor beta 1
Database Link	Entrez Gene 7040 Human UniProt ID P01137
Synonyms	TGFB, Transforming growth factor beta-1, TGF-beta-1
Reference Data

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