VEGFA Mouse Monoclonal Antibody [Clone ID: 4B2-8]

Specifications

Product Data
Clone Name	4B2-8
Applications	ELISA, WB
Recommend Dilution	ELISA: In a Sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human VEGF165 when used with Biotin anti-Human VEGF165 antibody (Cat.-No PP1073B) as the detection antibody at a concentration of approximately 0.25-1.0 μg/ml. Western Blot: To detect Human VEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human VEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human VEGF165 (Cat.-No PA136).
Specificity	This antibody detects Vascular Endothelial Growth Factor. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction from cell culture
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	vascular endothelial growth factor A
Database Link	Entrez Gene 7422 Human UniProt ID P15692
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Reference Data

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