Resistin (RETN) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 0.5 µg/ml of this antibody will detect at least 150 pg/ml of recombinant Human Resistin when used with Biotin anti-Human Resistin antibody (Cat.-No. PP1214B) as the detection antibody at a concentration of at least 0.5-1.0 µg/ml. Western Blot: To detect Human Resistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Resistin is 0.20-0.40 ng/lane, under non-reducing conditions.  Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. Recommended concentration: 1.0 μg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human Resistin (Cat.-No PA205)
Specificity	This antibody detects Human Resistin. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml
Purification	Purified from cell culture by Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	resistin
Database Link	Entrez Gene 56729 Human UniProt ID Q9HD89
Background	Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.
Synonyms	RETN, FIZZ3, HXCP1, RSTN, ADSF
Reference Data

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