Bone Sialoprotein (IBSP) (native) Mouse Monoclonal Antibody [Clone ID: ID1.2]

Specifications

Product Data
Clone Name	ID1.2
Applications	ELISA, IF, IHC, R, WB
Recommend Dilution	ELISA (Cogan 2004). RIA. Western blot: 1/1,000 (Wuttke 2001, Cogan 2004, Carlinfante2003). Immunocytochemistry: 1/200 (Cogan 2004, Hilbig 2007, Graf 2012). Immunohistochemistry on Paraffin Sections: 1/400-1/2000. Immunohistochemistry on Cryo Sections, Resin-Embedded Sections:  1/100 (Ibrahim 2000, Cogan 2004, Scherberich 2007,Bauerle 2005, Mittag 2012, Carlinfante 2003, Amir 2007).
Reactivity	Human, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Bone Sialoprotein (Bone extract).
Specificity	This antibody recognizes Human Bone Sialoprotein, Recombinant Human, Recombinant Porcine and Recombinant Rat BSP Rabbit BSP. There was no cross reactivity obtained with Human, Porcine Osteonectin and Osteopontin.
Formulation	50mM TRIS, pH 7.4 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 10 µl aqua bidest to 1 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	integrin binding sialoprotein
Database Link	Entrez Gene 3381 Human UniProt ID P21815
Background	The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12% of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
Synonyms	Bone sialoprotein II, BSP II, IBSP, BNSP
Note	LocusID 3381
Reference Data

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