VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]

CAT#: AM03029FC-N

VCP mouse monoclonal antibody, clone Hs-14, FITC

Conjugation: Unconjugated FITC



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CNY 5,000.00


货期*
5周

规格
    • 50 Tests

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Specifications

Product Data
Clone Name Hs-14
Applications FC, IF
Recommend Dilution Suitable for Flow Cytometry analysis of sperm cells using 20 μl reagent / 100 μl cell suspension.
The content of a vial (1 ml) is sufficient for 50 tests.
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid, 10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0.2% Acetic Acid and subsequently used for immunization.
Specificity The antibody  reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Formulation Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2% (w/v) high-grade BSA (protease free) as stabilizer
Label: FITC
State: Liquid purified Ig fraction
Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Conjugation FITC
Storage Condition Store the antibody in the dark at 2-8°C.
DO NOT FREEZE!
Avoid prolonged exposure to light.
Gene Name valosin containing protein
Background VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Synonyms 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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