CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LT95]

Specifications

Product Data
Clone Name	LT95
Applications	FC, IHC
Recommend Dilution	Flow Cytometry. Immunohistochemistry on Paraffin Sections. Positive tissue: tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HUT-78 Human T cell lymphoma cell line.
Specificity	The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Protein-A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	Fas cell surface death receptor
Database Link	Entrez Gene 355 Human UniProt ID P25445
Background	CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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