Clusterin (CLU) Mouse Monoclonal Antibody [Clone ID: Hs-3]

Specifications

Product Data
Clone Name	Hs-3
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA. Western blot: 1/ µg/ml Positive Material: seminal plasma Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive Tissue: Sertoli cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity	Recognizes Apolipoprotein J (Apo J).
Formulation	PBS, pH~7.4 with 15 mM Sodium Azide as preservative State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	clusterin
Database Link	Entrez Gene 1191 Human UniProt ID P10909
Background	Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.
Synonyms	Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40,40
Reference Data

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