HLAG (HLA-G) (C-term) Mouse Monoclonal Antibody [Clone ID: 2A12]

Specifications

Product Data
Clone Name	2A12
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: excellent for intracellular staining; 1-10 µg/ml. Western Blot. Immunohistochemistry (paraffin and frozen sections).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin
Specificity	The antibody 2A12 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Formulation	PBS, pH 7.4 with 15 mM sodium azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	major histocompatibility complex, class I, G
Database Link	Entrez Gene 3135 Human UniProt ID P17693
Background	HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
Synonyms	HLA-6.0, HLAG, MHC class I antigen G
Reference Data

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