Hsp60 (HSPD1) (1-573) Mouse Monoclonal Antibody [Clone ID: 2E4]

Specifications

Product Data
Clone Name	2E4
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Western blot: 1/1,000-1/2,000. Immunohistochemistry on Paraffin Embedded Sections: 1/50-1/100 (following antigen retrieval and detection by DAB). Flow cytometry. Immunocytochemistry / Immunoflourescence.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human HSP60 (1-573 aa) purified from E. coli
Specificity	This antibody detects Human HSP60 at aa 1-573. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein family D (Hsp60) member 1
Database Link	Entrez Gene 3329 Human UniProt ID P10809
Background	Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.
Synonyms	HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60
Reference Data

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