CD69 Mouse Monoclonal Antibody [Clone ID: FN50]

Specifications

Product Data
Clone Name	FN50
Applications	FC, FN, IHC, WB
Recommend Dilution	Flow Cytometry: 5 µg/ml. Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa. Immunohistochemistry on Frozen Sections. Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together with TPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheral blood B cells has similar, but weaker effect.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Anti-µ-stimulated human B lymphocytes
Specificity	The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD69 molecule
Database Link	Entrez Gene 969 Human UniProt ID Q07108
Background	CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
Synonyms	GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM
Reference Data

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