CD22 Mouse Monoclonal Antibody [Clone ID: IS7]

Specifications

Product Data
Clone Name	IS7
Applications	FC
Recommend Dilution	Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10e6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Reh human cell line
Specificity	This antibody reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
Formulation	Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent Label: APC State: Liquid Ig fraction Label: Conjugated with cross-linked Allophycocyanin under optimum
Purification	Size-exclusion chromatography
Conjugation	APC
Storage Condition	Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD22 molecule
Database Link	Entrez Gene 933 Human UniProt ID P20273
Background	CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Synonyms	SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM
Reference Data

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