CD2 Mouse Monoclonal Antibody [Clone ID: T6.3]

Specifications

Product Data
Clone Name	T6.3
Applications	FC
Recommend Dilution	Suitable for Monitoring of T cells in peripheral blood, Analysis of NK subsets, Study of T cell activation. Flow cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
Specificity	This antibody is analyzed in multi-color with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
Formulation	PBS with 0.08% sodium azide as preservative and 0.2% protein carrier. Label: APC State: Liquid IgG fraction. Label: Allophycocyanin
Concentration	lot specific
Conjugation	APC
Storage Condition	Store the antibody (undiluted) at 2-8°C. Do Not Freeze!
Gene Name	CD2 molecule
Database Link	Entrez Gene 914 Human UniProt ID P06729
Background	Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.
Synonyms	SRBC, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor
Note	Protocol: PBMC: Add 10 ul of Anitbody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a Flow cytometer or microscope.
Reference Data

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