CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: OFYS]
CAT#: AM05207PU-N
CD11a (ITGAL) mouse monoclonal antibody, clone OFYS, Purified
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CNY 4,200.00
货期*
4周
规格
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Specifications
Product Data | |
Clone Name | OFYS |
Applications | FC |
Recommend Dilution | Flow Cytometry. Characterization of LFA-1 immunodeficiency. Monitoring of subsets in peripheral blood. Graft-versus-host disease. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Specificity | This antibody reacts with LFA-1 alpha. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity. |
Formulation | PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Gene Name | integrin subunit alpha L |
Database Link | |
Background | CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion. |
Synonyms | Integrin alpha-L, LFA1, LFA-1 |
Note | Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
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