CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: F6.2]
CAT#: AM05208BT-N
CD11b (ITGAM) mouse monoclonal antibody, clone F6.2, Biotin
Conjugation: Unconjugated FITC
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CNY 4,435.00
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Specifications
Product Data | |
Clone Name | F6.2 |
Applications | FC |
Recommend Dilution | Flow Cytometry. Monitoring of subsets in peripheral blood. Characterization of leukemias and lymphomas. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Formulation | Sterile filtered PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative Label: Biotin State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Biotin |
Storage Condition | Store undiluted at 2-8°C. DO NOT FREEZE! |
Gene Name | integrin subunit alpha M |
Database Link | |
Background | (Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes. |
Synonyms | ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1 |
Note | Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
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