Calpain 1 (CAPN1) Mouse Monoclonal Antibody [Clone ID: 15C10]

Specifications

Product Data
Clone Name	15C10
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 0.5-1 µg/ml. Western blot: 0.5-1 µg/ml. Immunoprecipitation of both the native and denatured protein: 1-2 µg/ml. This Clone has been described to be suitable for Immunohistochemistry on Paraffin Sections.
Reactivity	Bovine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Calpain purified from Human placenta.
Specificity	This antibody (large subunit, p80) recognizes the 80 kD subunit of µ-Calpain as well as two smaller proteins that are presumed to be autolysis products.
Formulation	20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction (> 95% pure).
Concentration	lot specific
Purification	Standard chromatographic techniques.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	calpain 1
Database Link	Entrez Gene 823 Human UniProt ID P07384
Background	The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Synonyms	Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1
Note	Predicted Molecular Weight: 80 kDa
Reference Data

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