Cytokeratin 14 (KRT14) Mouse Monoclonal Antibody [Clone ID: DE-SPK14]

Specifications

Product Data
Clone Name	DE-SPK14
Applications	IHC, WB
Recommend Dilution	Western Blot: Detects Human Cytokeratin 14 at 50 kDa. Immunohistochemistry on Frozen Sections: It provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas. Immunohistochemistry on Paraffin-Embedded Tissue when using the Microwave method.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of Human Cytokeratin 14 (KVVSTHEQVLRTKN)­
Specificity	This antibody reacts specifically with Human Cytokeratin-14 (50 kDa) by Immunoblotting. Does not react with other Cytokeratins or other proteins.
Formulation	PBS State: Purified State: Liquid (sterile filtered) purified IgG fraction. Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	keratin 14
Database Link	Entrez Gene 3861 Human UniProt ID P02533
Background	Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.
Synonyms	Cytokeratin-14, CK14, Keratin 14, K14, KRT14
Reference Data

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