ATIC Mouse Monoclonal Antibody [Clone ID: F38 P7 H9]

Specifications

Product Data
Clone Name	F38 P7 H9
Applications	IHC, WB
Recommend Dilution	Western Blot: 1-2 µg/ml. Immunohistochemistry on Paraffin Sections (15 µg/ml). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Reactivity	Drosophila, Frog, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human ATIC protein and mouse myeloma Ag8563 cells. Sequence common in Frog, Fruit fly, Rat and Mouse.
Specificity	This antibody detects Phosphoribosylaminoimidazolecarboxamide Formyltransferase (ATIC).
Formulation	Phosphate buffered saline with 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C only. Avoid repeated freezing and thawing.
Gene Name	5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Database Link	Entrez Gene 471 Human UniProt ID P31939
Background	The bifunctional purine biosynthesis protein PURH contains phosphoribosylaminoimidazole carboxamide formyltransferase, also designated AICAR transformylase, IMP cyclohydrolase or Inosinicase. AICAR plays an important role in purine biosynthesis, specifically in the production of nucleotides and IMP. Defects in ATIC, the gene encoding for this protein, can cause AICArebosuria, also designated AICA-ribosiduria, an inborn error in purine biosynthesis that is neurologically cataclysmic. Individuals with AICA-rebosuria accumulate AICA-ribotide, also designated ZMP, and its derivatives in erythrocytes and fibroblasts and also excrete very large amounts of AICA-riboside in the urine. Mental retardation, epilepsy, dysmorphic features and congenital blindness are all symptoms of this disease.
Synonyms	IMP cyclohydrolase, Inosinicase, IMP synthetase
Reference Data

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