P Glycoprotein (ABCB1) Mouse Monoclonal Antibody [Clone ID: UIC2]

Specifications

Product Data
Clone Name	UIC2
Applications	FC, IF, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/20-1/200 diluted MDR1/CD243 antibody to label 1x106 cells in 100 µl. . Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200. This product may benefit from antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose (Re.3,4). Positive Control: Human Kidney.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
Specificity	This Monoclonal antibody specifically recognises an extracellular conformational epitope of CD243. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell (See Ref.2). Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. We recommend the use of Azide Free CD243 Antibody for Functional Studies.
Formulation	Phosphate buffered saline pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily B member 1
Database Link	Entrez Gene 5243 Human UniProt ID P08183
Background	CD243 is also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists.
Synonyms	Multidrug resistance protein 1, P-glycoprotein 1, ABCB1, PGY1
Reference Data

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