CD11R1 Mouse Monoclonal Antibody [Clone ID: MIL4]

Specifications

Product Data
Clone Name	MIL4
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 1x106 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Reactivity	Guinea Pig, Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine Lamina Propria Leucocytes
Specificity	This antibody recognizes the cell surface antigen classified as CD11R1 and stains Porcine eosinophils, a subset of neutrophils and NK cells. The CD11R1 antigen is a 165kD molecule, identical in size to Human CD11b. However, the expression pattern of CD11b in the Human and CD11R1 in the pig are different. Clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994). Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of 95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of 165kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001). Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Reference Data

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