IFNG Mouse Monoclonal Antibody [Clone ID: CC330]

Specifications

Product Data
Clone Name	CC330
Applications	ELISA, FC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells or 100 µl whole blood (Membrane permeabilisation is required). ELISA: 5 µg/ml as Capture antibody. This antibody may be used in combination with clone CC302 (Cat#AM05867BT-N) and Recombinant Bovine IFN gamma in a Sandwich ELISA or ELISPOT assays.
Reactivity	Bovine, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant bovine IFN-gamma.
Specificity	This antibody recognises Interferon gamma.
Formulation	Phosphate buffered saline with 0.09% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 281237 Bovine UniProt ID P07353
Background	Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms	IFN-gamma, gamma IFN
Reference Data

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