Aspergillus Mouse Monoclonal Antibody [Clone ID: WF-AF-1]

Specifications

Product Data
Clone Name	WF-AF-1
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA. Immunoprecipitation. Western Blot: Detects a band of approximately 106 kDa of Aspergillus fumigatus wall fraction (WF). Immunohistochemistry on Paraffin Sections: 1/300. Requires protein digestion pre-treatment (See Jensen et al. (2000) for more details). Positive Control: Aspergillus infected placenta Tissue.
Reactivity	Aspergillus
Host	Mouse
Clonality	Monoclonal
Immunogen	Wall fraction (WF) of Aspergillus fumigatus. Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Specificity	This antibody raised against the wall fraction (WF) of Aspergillus fumigatus specifically recognises members of the Aspergillus spp. including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae. Clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium. Clone WF-AF-1 has been successfully used in Immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in Human tissue sections.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgM fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulfate Precipitation
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing.
Background	The genus Aspergillus includes over 185 species. Around 20 species have been reported as causative agents of opportunistic infections in humans. Among these, Aspergillus fumigatus is the most commonly isolated species, followed by Aspergillus flavus. Aspergillus fumigatus is the major cause of aspergillosis. This organism causes both invasive and allergic aspergillosis. Aspergillus also produce fungal toxins called mycotoxins. Aflatoxin is produced by Aspergillus flavus as it grows on corn and peanuts. The toxin is poisonous to Humans by ingestion and causes liver disease. Aspergillus nidulans can produce the mycotoxin sterigmatocystin. This toxin has been shown to produce liver and kidney damage in lab animals. Aspergillus oschraceus,found in grains, soil and salted food products can produce a kidney toxin called oschratoxin A, which may produce oschratoxicosis in humans. Ochratoxin may also be produced by other aspergillus and penicillium species. Other toxins that can be produced by this fungus include penicillic acid, xanthomegnin and viomellein. Aspergillus infections have a very high mortality rate. Their incidence is growing because of the increased number of immunocompromised patients. Previous to antibodies such as these, special stains were used to identify aspergillus. Aspergillus oryzae and Aspergillus niger are used extensively in industrial scale fermentation to produce enzymes for processing household food and drink products. A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle.
Reference Data

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